Biomarker by T. Khan

By T. Khan

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The IPSS considers the percentage of bone marrow blasts, the number of peripheral blood cytopenias and the cytogenetic, the prognostic factors most important in relation to survival time and about the rate of leukemic transformation. The IPSS recognized four risk groups: low risk, intermediate 1, intermediate 2 and high risk. This system considers three categories for cytogenetic analysis: low risk [normal karyotypes, -Y, del(5q) and del(20q)]; high risk (alterations involving chromosome 7 and complex karyotypes) and intermediate risk (other chromosome abnormalities) .

K 1 2 3 4 Any distribution no information ≥ 75% ≥ 88% ≥ 93,75 % Normal distribution 68% 95% 99,7% 99,9% Table 4. Chebyshev’proportions compared with proportions of a normal distribution. If we decide to approximate clinical measurements by a normal curve, we are deciding to use a parametric hyphotesis test. A hypothesis test asks if an effect (difference) exists or not using statistics tests to verify the hypothesis that there is no difference. This is the null hypothesis and designated H0. The hypothesis that contradicts H0 is the alternative hypothesis and written HA.

In addition, MBD proteins are frequently found associated with histone deacetylases. Physiological DNA methylation has been shown important to regulate genetic expression during embryonic development, genomic imprinting, X chromosome inactivation and cancer (Worn & Gulberg, 2002). Chromatin is defined by a DNA and DNA-associated proteins, known as histones, in which genomic eukaryotic DNA is packaged. The basic unit of chromatin is called nucleosome, which is composed of a small DNA sequence, approximately 147 bases pairs, wrapped on 22 Biomarker protein octamer of the four core Histones (H2A, H2B, H3, and H4) and linker Histone H1.

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