Biological NMR Spectroscopy by John L. Markley, Stanley J. Opella

By John L. Markley, Stanley J. Opella

This e-book offers a severe review of development at the use of nuclear magnetic resonance spectroscopy to figure out the constitution of proteins, together with short stories of the background of the sector in addition to assurance of present scientific and in vivo functions. The publication, in honor of Oleg Jardetsky, one of many pioneers of the sphere, is edited through of the main hugely revered investigators utilizing NMR, and contours contributions by means of many of the prime employees within the box.

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Natl. Acad. Sci. A. , and Jardetzky, O. (1967). Proc. Natl. Acad. Sci. A. 5 8, 2212. , and Jardetzky, O. (1968). Biochemistry 7, 1226. , and Jardetzky, O. (1970). Adv. Prot. Chem. 2 4, 447. G. (1957). J. Am. Chem. Soc. 7 9, 3289. , and Jardetzky, O. (1957). J. Chem. Phyx. 2 6, 1346. , and Jardetzy, O. (1956). J. Chem. Phys. 2 5, 357. G. Shulman MR Center Yale University New Haven, CT 06520 USA It was my pleasure to participate in Oleg's 65th birthday celebration and to reminisce about the early days of Biochemical NMR.

R. Gryk and O. , 1981; Heatwole and Somerville, 1991, 1992). The ligand-free form (aporepressor) shows only weak binding (KD ~ 106 - 107 M) to DNA, independent of the nucleotide sequence (Carey, 1988; Hurlburt and Yanofsky, 1990). , 1989; Hurlburt and Yanofsky, 1990). , 1973). With a molecular weight of approximately 25kD, the trp repressoris one of the smallest regulatory systems known, which makes it attractive as a prototype for the study of the molecular mechanism of allosteric regulation. In the twelve years since it was isolated and purified (Joachimiak et al, 1983), it has become one of the most extensively studied allosteric systems.

The structure of the DNA binding domains has several interesting features. In the crystal structures, the residues implicated in DNA binding from homology studies (Ohlendorf et al, 1983) and from mutational analysis (Kelley and Yanofsky, 1985) were indeed observed to adopt a helix-turn-helix conformation as predicted, although the isotropic temperature factors for the DE helices were somewhat larger than those found for core residues (Lawson et al, 1988). The solution structures of the repressors present a similar overall picture of the DNA binding helices, when considering the effects that dynamic fluctuations have on the average structure.

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