Introduction to Medical Laboratory Technology by F. J. Baker

By F. J. Baker

Accomplished undergraduate textbook at the ideas and perform of labor in scientific laboratories. incorporates a common creation, and chapters on: scientific chemistry,cellular pathology, microbiology, hematology, and transfusion technological know-how. Halftone illustrations. earlier version: c1985. Softcover. DNLM: Laboratory options and tactics.

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In other words empty magnification is magnification without resolution. SETTING UP THE MICROSCOPE I f the best results are to be obtained it is of the utmost importance that the microscope is set up correctly. Two methods of adjusting the illumination, the Nelson and Köhler methods, are in universal use. O f these methods, the latter is rapidly becoming the more widely used and research microscopes equipped with built-in illu­ mination are designed to be adjusted for Köhler illumination. T h e term 'critical illumination' was coined during the latter part of the last century by E .

Nelson, the famous British critical microscopist. T h e method was devised in order to obtain maxi­ mum resolution and to achieve this even illumination of the whole field was sacrificed. An image of the light source is focused in the ELEMENTARY MICROSCOPY 51 object plane, resulting in the object under examination being illuminated by a solid cone of light. 24). 24. Diagram illustrating critical illumination (Nelson). The light source S is imaged in the plane of the object Ο by the sub-stage condenser C.

Most micro­ scope lamps use electricity as the source of energy and good lamps possess the following features. T h e base is of sufficient size and weight to ensure perfect stability. T h e lamphouse has good ventilation to prevent over­ heating, but is light-tight, except for the working orifice, and is supported on a stand which permits smooth adjustment of the height. Facilities are provided which permit adjustment in both a vertical and horizontal direction, and provision is made for coloured filters to be interposed in the light path.

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